KMID : 0352720230470060773
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Journal of Ginseng Research 2023 Volume.47 No. 6 p.773 ~ p.783
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Botrytis cinerea hypovirulent strain ¡âBcSpd1 induced Panax ginseng defense
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Shuhan Zhang
Junyou Han Ning Liu Jingyuan Sun Huchen Chen Jinglin Xia Huiyan Ju Shouan Liu
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Abstract
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Background : Gray mold, caused by Botrytis cinerea, is one of the major fungal diseases in agriculture. Biological methods are preferred over chemical fungicides to control gray mold since they are less toxic to the environment and could induce the resistance to pathogens in plants. In this work, we try to understand if ginseng defense to B. cinerea could be induced by fungal hypovirulent strain ¡âBcSpd1. BcSpd1 encodes Zn(II)2Cys6 transcription factor which regulates fungal pathogenicity and we recently reported ¡âBcSpd1 mutants reduced fungal virulence.
Methods : We performed transcriptomic analysis of the host to investigate the induced defense response of ginseng treated by B. cinerea ¡âBcSpd1. The metabolites in ginseng flavonoids pathway were determined by UPLC-ESI-MS/MS and the antifungal activates were then performed.
Results : We found that ¡âBcSpd1 enhanced the ginseng defense response when applied to healthy ginseng leaves and further changed the metabolism of flavonoids. Compared with untreated plants, the application of ¡âBcSpd1 on ginseng leaves significantly increased the accumulation of p-coumaric acid and myricetin, which could inhibit the fungal growth.
Conclusion : B. cinerea ¡âBcSpd1 could effectively induce the medicinal plant defense and is referred to as the biological control agent in ginseng disease management.
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KEYWORD
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Botrytis cinerea, Panax ginseng, p-coumaric acid, myricetin, transcriptome
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